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High-Fat Western Diet Alters Silica-induced airway epithelium ion exchange but not airway smooth muscle reactivity_dataset
Updated January 8, 2024
January 2024
NIOSH Dataset RD-1068-2023-0
Introduction
Silicosis is an irreversible occupational lung disease resulting from crystalline silica exposure. Previously, we discovered that Western diet (HFWD)-consumption increases susceptibility to silica-induced pulmonary inflammation and fibrosis. This study investigates the potential of HFWD to alter silica-induced effects on airway epithelial ion transport and smooth muscle reactivity.
General description of data collection methods
This was a laboratory-based investigation that utilized a rat model to study the effects of a high-fat Western diet on silica-induced changes in airway epithelial ion transport and airway smooth muscle reactivity. Six-week-old male F344 rats were fed a HFWD or standard rat chow (STD) and exposed to silica (Min-U-Sil 5®, 15 mg/m3, 6 h/d, 5 d/wk, for 39 d) or filtered air. Experimental endpoints were measured at 0, 4, and 8 wk post-exposure. Transepithelial potential difference (Vt), short-circuit current (ISC) and transepithelial resistance (Rt) were measured in tracheal segments and ion transport inhibitors [amiloride, Na+ channel blocker; NPPB; Clˉ channel blocker; ouabain, Na+, K+-pump blocker] identified changes in ion transport pathways. Changes in airway smooth muscle reactivity to methacholine (MCh) were investigated in the isolated perfused trachea.
Publication based on the data set
Thompson, JA, Kashon, ML, McKinney, W, and Fedan, JS. High-Fat Western Diet Alters Silica-induced airway epithelium ion exchange but not airway smooth muscle reactivity. BMC Res Notes. 2024 Jan 3;17(1):13. doi: 10.1186/s13104-023-06672-w. PMID: 38172968.
Acknowledgement
When a publication makes use of this data set, acknowledgement of the development of the data set should be attributed to National Institute for Occupational Safety and Health (NIOSH), Health Effects Laboratory Division (HELD). This project was funded by NIOSH intramural NORA funding of the project, “Health Effects of Inhaled Crude,” CAN 9390DT3.
Contributors to this investigation were:
Janet A. Thompson
jsd7@cdc.gov
Michael L. Kashon
mqk1@cdc.gov
Walter McKinney
wdm9@cdc.gov
Jeffrey S. Fedan
jsf2@cdc.gov
Contact
For further information, contact:
Pathology and Physiology Branch
HELD
NIOSH