Toluene-2,4-diamine

RTECS #

XS9625000

CAS #

95-80-7
See: NMAM or OSHA Methods

Updated

December 2018

Molecular Weight

122.19

Molecular Formula

C₇H₁₀N₂

Synonyms

1,3-Benzenediamine, 4-methyl-
1,3-Diamino-4-methylbenzene
2,4-Diamino-1-methylbenzene
2,4-Diamino-1-toluene
2,4-Diaminotoluen (Czech)
2,4-Diaminotoluene
2,4-Diaminotoluol
2,4-Tolamine
2,4-Tolylenediamine
3-Amino-p-toluidine
4-Methyl-1,3-benzenediamine
4-Methyl-m-phenylenediamine
5-Amino-o-toluidine
Benzofur MT
Brown for Fur T
C.I. 76035
C.I. Oxidation Base 20
C.I. Oxidation Base 200
C.I. Oxidation Base 35
Developer 14
Developer B
Developer DB
Developer DBJ
Developer MC
Developer MT
Developer MT-CF
Developer MTD
Developer T
Eucanine GB
Fouramine
Fouramine J
Fourrine 94
Fourrine M
MTD
m-Toluenediamine
m-Toluylendiamin (Czech)
m-Toluylenediamine
m-Tolyenediamine
m-Tolylenediamine
Nako TMT
NCI-C02302
Pelagol Grey J
Pelagol J
Pontamine developer TN
RCRA waste number U221
Renal MD
TDA
Tertral G
Tolylene-2,4-diamine
Zoba GKE

Skin and Eye Irritation and References

Route/Organism	Dose	Effect	Reference
eye /rabbit	100 mg/24H	moderate	85JCAE -,477,1986
eye /rabbit	100 mg/24H		TAEHC* IP:74,1,1987
skin /rabbit	500 mg/24H		TAEHC* IP:75,1,1987
skin /rabbit	500 mg/24H	mild	85JCAE -,477,1986

Mutation Data and Reference

System Test	Route/Organism/Tissue	Dose	Reference
body fluid assay	Salmonella typhimurium/rat	70 mg/kg	JJIND8 76,291,1986
Cytogenetic Analysis	ovary/hamster	4 mmol/L	MUREAV 265,45,1992
DNA adduct	oral/rat	84 mg/kg/3W-continuous	MUREAV 367,209,1996
DNA Damage	intraperitoneal/rat	37 mg/kg	CRNGDP 6,1285,1985
DNA Damage	intraperitoneal/mouse	240 mg/kg	MUREAV 391,201,1997
DNA Damage	oral/mouse	60 mg/kg	MUREAV 440,1,1999
DNA Damage	fibroblast/human	100 µmol/L	MUREAV 127,107,1984
DNA Damage	mammary gland/human	98 mg/L/24H (-enzymatic activation step)	MUREAV 588,47,2005
DNA Damage	other cell types/human	1.46 mmol/L/20H	TXCYAC 213,138,2005
DNA Damage	liver/human	1.46 mmol/L/20H	TXCYAC 213,138,2005
DNA Damage	other cell types/human	0.316 mmol/l/48H	MUREAV 696,21,2010
DNA Damage	other cell types/human	31.6 mmol/l/48H	MUREAV 696,21,2010
DNA Damage	liver/human	0.316 mmol/l/48H	MUREAV 696,21,2010
DNA Damage	other cell types/mouse	0.125 mmol/L	MUREAV 715,25,2011
DNA Damage	oral/rat	1000 mg/kg/21H- intermittent	MUREAV 786-788,3,2015
DNA Damage	oral/rat	300 mg/kg/3D- intermittent	MUREAV 786-788,151,2015
DNA Damage	other cell types/human	1 µmol/L/24H	MUTAEX 31,83,2016
DNA Damage	other cell types/human	1 mmol/L/24H (+enzymatic activation step)	MUREAV 760,23,2014
DNA Damage	other cell types/mouse	625 µmol/L/24H	MUREAV 810,13,2016
DNA inhibition	intraperitoneal/mouse	111 mg/kg	MUREAV 91,75,1981
micronucleus test	liver/human	2.14 mmol/L/20H	TXCYAC 213,138,2005
micronucleus test	other cell types/human	2.14 mmol/L/20H	TXCYAC 213,138,2005
micronucleus test	oral/rat	700 mg/kg/14D- intermittent	MUREAV 747,234,2012
micronucleus test	oral/rat	700 mg/kg/28D- intermittent	MUREAV 747,234,2012
micronucleus test	other cell types/human	134 mg/L/3H (+enzymatic activation step)	MUREAV 767,28,2014
micronucleus test	lymphocyte/human	1222 mg/L/3H (+enzymatic activation step)	MUREAV 767,28,2014
micronucleus test	other cell types/human	316 µmol/L/3H	MUREAV 724,7,2011
micronucleus test	oral/rat	125 mg/kg	MUTAEX 24,9,2009
micronucleus test	oral/rat	125 mg/kg/2D- intermittent	MUREAV 698,24,2010
morphological transform	embryo/mouse	20 mg/L/72H	MUREAV 702,100,2010
morphological transform	embryo/hamster	50 mg/L/24H	MUREAV 577S,1,2005
morphological transform	fibroblast/mouse	10 mg/L/21D (-enzymatic activation step)	EMMUEG 35,300,2000
morphological transform	embryo/hamster	10 mg/L/7D	MUREAV 654,108,2008
morphological transform	embryo/hamster	500 µg/L	CALEDQ 3,45,1977
mutation in mammalian somatic cells	lymphocyte/mouse	198 mg/L	MUREAV 135,115,1984
mutation in mammalian somatic cells	ovary/hamster	2 gm/L	MUREAV 135,115,1984
mutation in microorganisms	/Salmonella typhimurium	625 µg/plate/20M (+enzymatic activation step)	MUTAEX 20,217,2005
mutation in microorganisms	/Salmonella typhimurium	2500 µg/plate/48H (+enzymatic activation step)	MUTAEX 20,217,2005
mutation in microorganisms	/Salmonella typhimurium	10 mg/plate (+enzymatic activation step)	ENMUDM 5(Suppl 1),3,1983
mutation in microorganisms	/Salmonella typhimurium	10 µg/plate (-enzymatic activation step)	JJIND8 76,291,1986
mutation in microorganisms	/Salmonella typhimurium	0.5 mmol/L/4H (+enzymatic activation step)	MUREAV 810,13,2016
other mutation test systems	intraperitoneal/mouse	200 mg/kg	MUREAV 747,164,2012
other mutation test systems	other cell types/human	500 µmol/L/24H	TIVIEQ 28,46,2014
other mutation test systems	/Salmonella typhimurium	60 mg/L	MUREAV 192,239,1987
other mutation test systems	ovary/hamster	6 mmol/L	MUREAV 265,45,1992
phage inhibition capacity	/Escherichia coli	100 mmol/L	MDMIAZ 31,11,1979
sex chromosome loss and nondisjunction	parenteral/Drosophila melanogaster	5 mmol/L	MUREAV 56,31,1977
sex chromosome loss and nondisjunction	oral/Drosophila melanogaster	15200 mmol/L/3D	MUREAV 48,181,1977
sister chromatid exchange	intraperitoneal/mouse	9 mg/kg	MUREAV 108,225,1983
specific locus test	other cell types/human	31.25 mg/L (-enzymatic activation step)	MUREAV 695,87,2010
specific locus test	skin/mouse	5600 mg/kg/28D-continuous	MUREAV 608,88,2006
specific locus test	other cell types/human	75 mg/L/16H (-enzymatic activation step)	MUREAV 724,76,2011
specific locus test	other cell types/human	16 mg/L/16H (+enzymatic activation step)	MUREAV 724,76,2011
specific locus test	other cell types/human	31.25 mg/L/48H (-enzymatic activation step)	MUTAEX 24,35,2009
specific locus test	other cell types/human	10 µmol/L/24H	EMMUEG 54,737,2013
Unscheduled DNA Synthesis	other cell types/human	0.01 mmol/L/20H	TXCYAC 213,138,2005
Unscheduled DNA Synthesis	liver/human	100 µmol/L	CNREA8 49,1075,1989
Unscheduled DNA Synthesis	oral/rat	150 mg/kg	ENMUDM 4,553,1982
Unscheduled DNA Synthesis	liver/rat	10 µmol/L	MUREAV 315,147,1994

Reproductive Effects Data and References

Route/Organism	Dose	Effect	Reference
oral/rat	1260 mg/kg (10W male)	Reproductive: Effects on fertility: Mating performance (e.g., # sperm positive females per # females mated; # copulations per # estrus cycles)	JTEHD6 16,753,1985
oral/rat	1260 mg/kg (10W male)	Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count) Reproductive: Paternal effects: Prostate, seminal vesicle, Cowper's gland, accessory glands	JTEHD6 16,763,1985
oral/rat	210 mg/kg (1W male)	Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count) Reproductive: Paternal effects: Testes, epididymis, sperm duct	JTEHD6 25,435,1988
oral/mouse	1200 mg/kg (6-13D pregnant)	Reproductive: Effects on fertility: Litter size (e.g., # fetuses per litter; measured before birth) Reproductive: Effects on newborn: Live birth index (Litter size (e.g., # fetuses per litter; measured after birth)	TCMUD8 7,29,1987

Tumorigenic Data and References

Route/Organism	Dose	Effect	Reference
oral/mouse	lowest published toxic dose: 8050 mg/kg/101W- continuous	Tumorigenic: Carcinogenic by RTECS criteria Liver: Tumors Blood: Lymphoma including Hodgkin's disease	NCITR* NCI-TR-162,1979
oral/mouse	toxic dose: 17 gm/kg/2Y- continuous	Tumorigenic: Carcinogenic by RTECS criteria Liver: Tumors Blood: Lymphoma including Hodgkin's disease	NCITR* NCI-TR-162,1979
oral/mouse	toxic dose: 8400 mg/kg/2Y- continuous	Tumorigenic: Carcinogenic by RTECS criteria Liver: Tumors	GANNA2 70,453,1979
oral/mouse	toxic dose: 16800 mg/kg/2Y- continuous	Tumorigenic: Carcinogenic by RTECS criteria Liver: Tumors	GANNA2 70,453,1979
oral/rat	lowest published toxic dose: 2100 mg/kg/90W- continuous	Tumorigenic: Carcinogenic by RTECS criteria Liver: Tumors Skin and Appendages: Tumors	JJIND8 62,1107,1979
oral/rat	toxic dose: 5030 mg/kg/84W- continuous	Tumorigenic: Carcinogenic by RTECS criteria Liver: Tumors Blood: Lymphoma including Hodgkin's disease	NCITR* NCI-TR-162,1979
oral/rat	toxic dose: 9000 mg/kg/36W- continuous	Tumorigenic: Carcinogenic by RTECS criteria Liver: Tumors	CNREA8 29,1137,1969

Acute Toxicity Data and References

Route/Organism	Dose	Effect	Reference
intraperitoneal/mouse	lethal dose (50 percent kill): 480 mg/kg		JEPTDQ 3(1-2),149,1979
intraperitoneal/rat	lethal dose (50 percent kill): 325 mg/kg		JEPTDQ 3(1-2),149,1979
oral/mouse	lowest published lethal dose: 380 mg/kg		MUREAV 747,164,2012
oral/rat	lethal dose (50 percent kill): 590 mg/kg/24H		TAEHC* -,1,1987
skin/rabbit	lethal dose (50 percent kill): 650 mg/kg/24H		TAEHC* -,1,1987
subcutaneous/Dog	lowest published lethal dose: 200 mg/kg		XPHBAO 271,56,1941
subcutaneous/rabbit	lowest published lethal dose: 400 mg/kg	Brain and Coverings: Other degenerative changes Behavioral: Convulsions or effect on seizure threshold Lung, Thorax, or Respiration: Respiratory stimulation	ZEPTAT 17,59,1915
subcutaneous/rat	lowest published lethal dose: 350 mg/kg	Behavioral: Somnolence (general depressed activity) Cardiac: Pulse rate decreased with fall in BP Lung, Thorax, or Respiration: Respiratory stimulation	ZEPTAT 17,59,1915

Other Multiple Dose Data and References

Route/Organism	Dose	Effect	Reference
oral/mouse	lowest published toxic dose: 700 mg/kg/7D- intermittent	Nutritional and Gross Metabolic: Weight loss or decreased weight gain	NTPTR* IMM-87034
oral/mouse	lowest published toxic dose: 1400 mg/kg/14D- intermittent	Liver: Hepatitis (hepatocellular necrosis), zonal Liver: Changes in liver weight Endocrine: Changes in spleen weight	NTPTR* IMM-87034
oral/mouse	lowest published toxic dose: 1400 mg/kg/14D- intermittent	Kidney, Ureter, and Bladder: Renal function tests depressed Blood: Changes in other cell count (unspecified) Blood: Changes in leukocyte (WBC) count	NTPTR* IMM-87034
oral/mouse	lowest published toxic dose: 1400 mg/kg/14D- intermittent	Immunological Including Allergic: Increase in cellular immune response Immunological Including Allergic: Decrease in cellular immune response Immunological Including Allergic: Decreased immune response	NTPTR* IMM-87034
oral/mouse	lowest published toxic dose: 1400 mg/kg/14D- intermittent	Immunological Including Allergic: Uncharacterized (multiple organ involvement) Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field	NTPTR* IMM-87034
oral/mouse	lowest published toxic dose: 700 mg/kg/14D- intermittent	Immunological Including Allergic: Increase in cellular immune response	NTPTR* IMM-87034
oral/rat	lowest published toxic dose: 725 mg/kg/29D- intermittent	Liver: Other changes	MUREAV 702,40,2010
oral/rat	lowest published toxic dose: 2900 mg/kg/29D- intermittent	Blood: Changes in platelet count Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field	MUREAV 702,40,2010
oral/rat	lowest published toxic dose: 700 mg/kg/28D- intermittent	Liver: Other changes	MUREAV 751,12,2013
oral/rat	lowest published toxic dose: 36500 mg/kg/2Y- intermittent	Kidney, Ureter, and Bladder: Other changes Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field	TAEHC* -,1,1987
oral/rat	lowest published toxic dose: 1050 mg/kg/70D - continuous	Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count) Reproductive: Paternal effects: Testes, epididymis, sperm duct Reproductive: Paternal effects: Prostate, seminal vesicle, Cowper's gland, accessory glands	TAEHC* IP:74,67,1987
oral/rat	lowest published toxic dose: 350 mg/kg/5D- intermittent	Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Multiple enzyme effects	TAEHC* -,1,1987
oral/rat	lowest published toxic dose: 732.48 mg/kg/16W- continuous	Liver: Tumors Liver: Changes in liver weight	TOXID9 78,367,2004
oral/rat	lowest published toxic dose: 2197.44 mg/kg/16W- continuous	Liver: Multiple effects Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol) Nutritional and Gross Metabolic: Weight loss or decreased weight gain	TOXID9 78,367,2004

Reviews

Organization	Standard	Reference
International Agency for Research on Cancer (IARC)	Cancer Review:Animal Sufficient Evidence	IMEMDT 16,83,1978
International Agency for Research on Cancer (IARC)	Cancer Review:Human No Adequate Data	IMEMDT 16,83,1978
International Agency for Research on Cancer (IARC)	Cancer Review:Group 2B	IMSUDL 7,56,1987
TOXICOLOGY REVIEW		MUREAV 543,201,2003
TOXICOLOGY REVIEW		MUREAV 567,227,2004
TOXICOLOGY REVIEW		EMMUEG 43,36,2004
TOXICOLOGY REVIEW		MUREAV 588,88,2005
TOXICOLOGY REVIEW		MUREAV 627,10,2007
TOXICOLOGY REVIEW		MUREAV 654,114,2008
TOXICOLOGY REVIEW		MUREAV 590,1,2005
TOXICOLOGY REVIEW		TXAPA9 243,239,2010
TOXICOLOGY REVIEW		MUREAV 705,184,2010
TOXICOLOGY REVIEW		MUTAEX 26,223,2011
TOXICOLOGY REVIEW		MUREAV 653,99,2008
TOXICOLOGY REVIEW		MUREAV 723,108,2011
TOXICOLOGY REVIEW		MUTAEX 27,257,2012
TOXICOLOGY REVIEW		MUREAV 769,34,2014
TOXICOLOGY REVIEW		FCTOD7 55,596,2013
TOXICOLOGY REVIEW		MUREAV 783,13,2015
TOXICOLOGY REVIEW		MUREAV 795,7,2016
TOXICOLOGY REVIEW		MUTAEX 31,117,2016
TOXICOLOGY REVIEW		MUREAV 802,1,2016
TOXICOLOGY REVIEW		RTOPDW 55,43,2009

Standards and Regulations

Organization	Standard	Reference
Occupational Exposure Limit-AUSTRIA	TRK-TMW 0.02 ppm (0.1 mg/m³);KZW 0.08 ppm (0.4 mg/m³), skin, sen, 2007
Occupational Exposure Limit-JAPAN	2B carc, MAY2012
Occupational Exposure Limit-POLAND	MAC(time-weighted average) 0.04 mg/m³, MAC(short term exposure limit) 0.1 mg/m³, JAN1999
Occupational Exposure Limit-RUSSIA	short term exposure limit 2 mg/m³, Skin, JUN2003
Occupational Exposure Limit-SWEDEN	Group B Carcinogen, Sen, JUN2005
Occupational Exposure Limit-SWITZERLAND	MAK-week 0.02 ppm (0.1 mg/m³), carc 2, skin, sen, JAN2011

NIOSH Documentation and Surveillance

Organization	Standard	Reference
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO TOLUENEDIAMINE-air	Carcinogen lowest feasible concentration	NIOSH* DHHS #92-100,1992
National Occupational Exposure Survey 1983	Hazard Code X5386; Number of Industries 6; Total Number of Facilities 130; Number of Occupations 15; Total Number of Employees Exposed 8511; Total Number of Female Employees Exposed 396

Status in Federal Agencies

Organization	Reference
EPA GENETOX PROGRAM 1988, Inconclusive: SHE-clonal assay
EPA GENETOX PROGRAM 1988, Negative: N crassa-aneuploidy; Sperm morphology-mouse
EPA GENETOX PROGRAM 1988, Positive: Carcinogenicity-mouse/rat; Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Positive: D melanogaster Sex-linked lethal
EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, PROPOSED RULE	FEREAC 47,27009,82
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NCI Carcinogenesis Bioassay (feed);clear evidence:mouse,rat	NCITR* NCI-TR-162,1979
NIOSH Analytical Method, 1994: 2,4-Toluenediamine, 5516
NIOSH CURRENT INTELLIGENCE BULLETIN 53, DECEMBER 1989
NTP 14th Report on Carcinogens,2016:Reasonably anticipated to be human carcinogen
NTP Carcinogenesis studies; test completed (peer review), October 2000
On EPA IRIS database
OSHA ANALYTICAL METHOD #ID-65

Page last reviewed: November 16, 2018

Content source: National Institute for Occupational Safety and Health